Fluorescent DNA in Optical Tweezers



A 10 kb DNA molecule labelled with digoxigenin and biotin is tethered between Streptavidin and anti-DIG coated microspheres held in double-trap optical tweezers. The DNA is stained with SYBR-gold and observed by epi-fluorescence imaging. The video shows many experiments, some with single DNA molecules between the beads, and some with two or more.
A brief glimpse of some surface-attached Qdots at the end.
Anders Wallin et al., 2009, University of Helsinki.


Post time: Jun-15-2017
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