Fluorescence microscopy movie shows seeds (labeled with Alexa 568), elongated MTs (labeled with Alexa 647) and Neutravidin beads (with DyLight 488). Each filament is attached to a single bead at the biotinylated seed region. Subsequently, the bottom MT is moved closer to the top one, and depletion-induced bundle spontaneously forms. Overlap region can be distinguished by increased brightness. Bottom MT is then moved up, thus changing the MT overlap.
Measuring Cohesion between Macromolecular Filaments One Pair at a Time: Depletion-Induced Microtubule Bundling F. Hilitski et al Physical Review Letters 114, 138102 (2015).
https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.114.138102
Post time: Jul-11-2017